YeasenTransfection

Hieff Trans™ Booster DNA/RNA Transfection Reagent

Polymer-based reagent for DNA and RNA delivery, supplied with a separate enhancer.

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Product information

Overview

Hieff Trans Booster is a polymer-based DNA/RNA transfection reagent supplied with a separate enhancer. YEASEN provides application data for cell lines and selected primary cells.

DNA and RNA types: DNA, mRNA, siRNA, miRNA and ASO
Kit contents: Booster reagent and an equal volume of enhancer
Enhancer use: DNA workflow; not required for mRNA or siRNA in the current manual
YEASEN test data: Cell lines and primary cells; DNA, mRNA and siRNA examples

Features and manufacturer data

Hieff Trans Booster is YEASEN's next-generation polymer-based DNA/RNA transfection reagent. The manufacturer describes a nucleic-acid-protective delivery design and provides data in established cell lines including HeLa and 293T, and selected primary or difficult-to-transfect models. Its listed payloads are DNA, mRNA, siRNA, miRNA and ASO. The kit supplies Booster reagent and an equal volume of a separate enhancer. Match the evidence to your payload and cell model before evaluation.

Broad DNA and RNA scope in one reagent family
Manufacturer application examples include primary and difficult-to-transfect cells
Separate enhancer supplied for the DNA workflow; the current manual does not require it for mRNA or siRNA
Application data available for a range of cell models

Manufacturer schematic comparing conventional transfection and Hieff Trans Booster stages: nucleic-acid loading, cellular entry, release and expression.

Manufacturer sources checked · 23 September 2026

Product page and linked manual checked on 23 September 2026. Manual revision: EN20260916. Manufacturer claims are not independent validation or a performance guarantee.

The September 2026 manual distinguishes enhancer use by payload. Do not transfer the RNAiBoost enhancer instructions to Booster.
DNA is a broad application category: the manual does not establish validation for every linear or closed-linear DNA format. Ask for topology- and cell-specific evidence where relevant.

Comparing another brand? Lipofectamine 3000 Alternative UK.

Source: YEASEN product specifications. Product information recorded on 12 August 2026. Follow the current manufacturer instructions. Product documents and sources.

Applications, specifications and components

Applications: Cell Transfection
DNA Transfection
mRNA/siRNA Transfection
Sales pack: 100 µL · 0.75 mL · 1.5 mL
Catalogue options: 40801ES01, 40801ES03, 40801ES04
Manufacturer: Yeasen
Manufacturer storage: Store at 2–8°C for up to one year. Follow the expiry date on the supplied batch.

Catalogue numbers, pack sizes and prices

Catalogue number	Pack size	Price ex VAT	Price incl. VAT
40801ES01	100 µL	£55.70	£66.84
40801ES03	0.75 mL	£264.67	£317.60
40801ES04	1.5 mL	£499.33	£599.20

Prices are in GBP. VAT-inclusive amounts use 20% VAT. Delivery is calculated at checkout. Select a pack size to check availability or request a quotation.

Specifications

Formulation	Polymer-based transfection reagent
Listed nucleic acids	DNA, mRNA, siRNA, miRNA and ASO
Supplied enhancer	Equal volume to Booster reagent
Enhancer use	DNA workflow; not required for mRNA or siRNA in manual EN20260916

Components

Component code	Name	40801 ES 01	40801 ES 03	40801 ES 04
40801-A	Booster DNA/RNA Transfection Reagent	100 μL	0.75 mL	1.5 mL
40801-B	Transfection Enhancer	100 μL	0.75 mL	1.5 mL

Application data and case studies

YEASEN application data

Find your cell model, DNA or RNA type. Open a case to see the figures and test conditions.

37 of 37

Cell line, model or nucleic acid

Select a case to view its data

High-Efficiency DNA TransfectionApplication figures
Application figures
High-Efficiency DNA Transfection
Open case link for High-Efficiency DNA Transfection (opens in a new tab)
Figure 1. DNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000.
High-Efficiency mRNA TransfectionApplication figures
Application figures
High-Efficiency mRNA Transfection
Open case link for High-Efficiency mRNA Transfection (opens in a new tab)
Figure 2. mRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000.
High-Efficiency siRNA TransfectionApplication figures
Application figures
High-Efficiency siRNA Transfection
Open case link for High-Efficiency siRNA Transfection (opens in a new tab)
Figure 3. siRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000.
High-Efficiency Primary Dermal Fibroblasts TransfectionPublished customer cases
Published customer cases
High-Efficiency Primary Dermal Fibroblasts Transfection
Open case link for High-Efficiency Primary Dermal Fibroblasts Transfection (opens in a new tab)
Figure 4. Plasmid EGFR DNA transfection in primary dermal fibroblasts using Booster DNA&RNA Transfection Reagent versus L*3000.
High-Efficiency Porcine Alveolar Macrophages TransfectionPublished customer cases
Published customer cases
High-Efficiency Porcine Alveolar Macrophages Transfection
Open case link for High-Efficiency Porcine Alveolar Macrophages Transfection (opens in a new tab)
Figure 5. DNA transfection in primary Porcine Alveolar Macrophages (PAMs) using Booster DNA&RNA Transfection Reagent versus L*3000.
PAMsPlasmid DNA(8000 bp)
Primary and neural stem cells
PAMs (Primary Porcine Alveolar Macrophages)
Open case link for PAMs (Primary Porcine Alveolar Macrophages), case 1 (opens in a new tab)
Case
1
Figure
Figure 7
Cell type
PAMs
Nucleic acid type
Plasmid DNA(8000 bp)
Culture plate size
6-well plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 7. Transfection of plasmid DNA into primary porcine alveolar macrophages using Booster DNA&RNA Transfection Reagent versus L*3000.
View official case PDF · page 8
MPFsPlasmid DNA(6162 bp)
Primary and neural stem cells
MPFs (Mouse Primary Fibroblasts)
Open case link for MPFs (Mouse Primary Fibroblasts), case 2 (opens in a new tab)
Case
2
Figure
Figure 8
Cell type
MPFs
Nucleic acid type
Plasmid DNA(6162 bp)
Culture plate size
12-well plate
Nucleic acid amount
1 μg
Transfection reagent volume
2 μL
Figure 8. Transfection of plasmid DNA into mouse primary fibroblasts using Booster DNA&RNA Transfection Reagent versus A*8000.
View official case PDF · page 8
MPHsPlasmid DNA(9500bp)
Primary and neural stem cells
MPHs (Mouse Primary Hepatocytes)
Open case link for MPHs (Mouse Primary Hepatocytes), case 3 (opens in a new tab)
Case
3
Figure
Figure 9
Cell type
MPHs
Nucleic acid type
Plasmid DNA(9500bp)
Culture plate size
3.5 cm dish
Nucleic acid amount
0.5 μg
Transfection reagent volume
1 μL
Figure 9. Transfection of plasmid DNA into Mouse liver primary cells using Booster DNA&RNA Transfection Reagent versus L*3000.
View official case PDF · page 9
mNSCsmRNA
Primary and neural stem cells
mNSCs (Mouse Primary Neural Stem Cells)
Open case link for mNSCs (Mouse Primary Neural Stem Cells), case 4 (opens in a new tab)
Case
4
Figure
Figure 10
Cell type
mNSCs
Nucleic acid type
mRNA
Culture plate size
96-well plate
Nucleic acid amount
20 ng
Transfection reagent volume
0.1 μL
Figure 10. Transfection of mRNA into Mouse primary neural stem cells using Booster DNA&RNA Transfection Reagent versus B*8008. The results showed that the transfection efficiency of Yeasen Booster transfection reagent was close to 100%.
View official case PDF · page 9
PSFsPlasmid DNA(2112 bp)
Primary and neural stem cells
PSFs (Primary Skin Fibroblasts)
Open case link for PSFs (Primary Skin Fibroblasts), case 5 (opens in a new tab)
Case
5
Figure
Figure 11
Cell type
PSFs
Nucleic acid type
Plasmid DNA(2112 bp)
Culture plate size
6-well plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 11. Transfection of plasmid DNA into Primary skin fibroblasts using Booster DNA&RNA Transfection Reagent. The result showed that Yeasen Booster transfection reagent can efficiently transfect.
View official case PDF · page 10
HPAECsPlasmid DNA(967 bp)
Primary and neural stem cells
HPAECs (Primary Human Pulmonary Artery Endothelial Cells)
Open case link for HPAECs (Primary Human Pulmonary Artery Endothelial Cells), case 6 (opens in a new tab)
Case
6
Figure
Figure 12
Cell type
HPAECs
Nucleic acid type
Plasmid DNA(967 bp)
Culture plate size
14-well plate
Nucleic acid amount
1 ug
Transfection reagent volume
2.5 μL
Figure 12. Transfection of plasmid DNA into Primary human pulmonary artery endothelial cells under hypoxic and normal conditions using Booster DNA&RNA Transfection Reagent. The result showed that Yeasen Booster transfection reagent can efficiently transfect.
View official case PDF · page 10
BMDMssiRNA
Primary and neural stem cells
BMDMs (Primary Bone Marrow-Derived Macrophages)
Open case link for BMDMs (Primary Bone Marrow-Derived Macrophages), case 7 (opens in a new tab)
Case
7
Figure
Figure 13
Cell type
BMDMs
Nucleic acid type
siRNA
Culture plate size
6-well-plate
Nucleic acid amount
50 nM
Transfection reagent volume
5 μL
Figure 13. siRNA transfection in primary bone marrow–derived macrophages using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent. Western blot results show that Yeasen Booster achieves more efficient gene knockdown.
View official case PDF · page 11
CEFsPlasmid DNA(4700bp)
Primary and neural stem cells
CEFs (Primary Chicken Embryo Fibroblasts)
Open case link for CEFs (Primary Chicken Embryo Fibroblasts), case 8 (opens in a new tab)
Case
8
Figure
Figure 14
Cell type
CEFs
Nucleic acid type
Plasmid DNA(4700bp)
Culture plate size
24-well-palte
Nucleic acid amount
0.5 μg
Transfection reagent volume
1 μL
Figure 14. Plasmid DNA transfection in primary chicken embryo fibroblasts using Booster DNA&RNA Transfection Reagent versus L*3000 transfection reagent.
View official case PDF · page 11
THP-1Plasmid DNA(6000 bp)
Suspension cells
THP-1 (Human Monocytic Leukaemia Cells)
Open case link for THP-1 (Human Monocytic Leukaemia Cells), case 9 (opens in a new tab)
Case
9
Figure
Figure 15
Cell type
THP-1
Nucleic acid type
Plasmid DNA(6000 bp)
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 15. Plasmid DNA transfection in THP-1 using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 12
JurkatPlasmid DNA（6000bp）
Suspension cells
Jurkat (Human T-Lymphocyte Leukaemia Cells)
Open case link for Jurkat (Human T-Lymphocyte Leukaemia Cells), case 10 (opens in a new tab)
Case
10
Figure
Figure 16
Cell type
Jurkat
Nucleic acid type
Plasmid DNA（6000bp）
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 16. Plasmid DNA transfection in Jurkat using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 12
Sf9Plsmid DNA(6738bp)
Suspension cells
Sf9 (Insect Ovarian Cells)
Open case link for Sf9 (Insect Ovarian Cells), case 11 (opens in a new tab)
Case
11
Figure
Figure 17
Cell type
Sf9
Nucleic acid type
Plsmid DNA(6738bp)
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 17. Plasmid DNA transfection in sf9 using Booster DNA&RNA Transfection Reagent versus L* 2000 transfection reagent.
View official case PDF · page 13
CHO-SPlasmid DNA(6000bp)
Suspension cells
CHO-S (Chinese Hamster Ovary Suspension Cells)
Open case link for CHO-S (Chinese Hamster Ovary Suspension Cells), case 12 (opens in a new tab)
Case
12
Figure
Figure 18
Cell type
CHO-S
Nucleic acid type
Plasmid DNA(6000bp)
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 18. Plasmid DNA transfection in CHO-S using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 13
A549Plasmid DNA(6000bp)
Tumour cells
A549 (Human Non-Small Cell Lung Carcinoma Cells)
Open case link for A549 (Human Non-Small Cell Lung Carcinoma Cells), case 13 (opens in a new tab)
Case
13
Figure
Figure 19
Cell type
A549
Nucleic acid type
Plasmid DNA(6000bp)
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 19. Plasmid DNA transfection in A549 using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 14
T24Plasmid DNA(9028bp)
Tumour cells
T24 (Human Bladder Transitional Cell Carcinoma Cells)
Open case link for T24 (Human Bladder Transitional Cell Carcinoma Cells), case 14 (opens in a new tab)
Case
14
Figure
Figure 20
Cell type
T24
Nucleic acid type
Plasmid DNA(9028bp)
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 20. Plasmid DNA transfection in T24 using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 14
HeLaPlasmid DNA(9500 bp)
Tumour cells
HeLa (Human Cervical Cancer Cells)
Open case link for HeLa (Human Cervical Cancer Cells), case 15 (opens in a new tab)
Case
15
Figure
Figure 21
Cell type
HeLa
Nucleic acid type
Plasmid DNA(9500 bp)
Culture plate size
24-well-plate
Nucleic acid amount
0.5 μg
Transfection reagent volume
1 μL
Figure 21. Plasmid DNA transfection in Hela using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 15
MC38Plasmid DNA(700bp)
Tumour cells
MC38 (Mouse Colon Cancer Cells)
Open case link for MC38 (Mouse Colon Cancer Cells), case 16 (opens in a new tab)
Case
16
Figure
Figure 22
Cell type
MC38
Nucleic acid type
Plasmid DNA(700bp)
Culture plate size
6-well-plate
Nucleic acid amount
5 μg
Transfection reagent volume
10 μL
Figure 22. Plasmid DNA transfection in Mc38 using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 15
AGSPlasmid DNA(2000bp)
Tumour cells
AGS (Human Gastric Adenocarcinoma Cells)
Open case link for AGS (Human Gastric Adenocarcinoma Cells), case 17 (opens in a new tab)
Case
17
Figure
Figure 23
Cell type
AGS
Nucleic acid type
Plasmid DNA(2000bp)
Culture plate size
10 cm dish
Nucleic acid amount
7.5 μg
Transfection reagent volume
15 μL
Figure 23. Plasmid DNA transfection in AGS using Booster DNA&RNA Transfection Reagent versus L* 2000 transfection reagent. The result shows that the L* 2000 reagent exhibits no visible difference between pre- and post-transfection (endogenous expression, bands shown with enhanced exposure), whereas Booster achieves a clear overexpression effect.
View official case PDF · page 16
HepG2Plasmid DNA(6000bp)
Tumour cells
HepG2 (Human Hepatocellular Carcinoma Cells)
Open case link for HepG2 (Human Hepatocellular Carcinoma Cells), case 18 (opens in a new tab)
Case
18
Figure
Figure 24
Cell type
HepG2
Nucleic acid type
Plasmid DNA(6000bp)
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 24. Plasmid DNA transfection in HepG2 using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 16
HCT-116siRNA
Tumour cells
HCT-116 (Human Colon Cancer Cells)
Open case link for HCT-116 (Human Colon Cancer Cells), case 19 (opens in a new tab)
Case
19
Figure
Figure 25
Cell type
HCT-116
Nucleic acid type
siRNA
Culture plate size
T25
Nucleic acid amount
50 nM
Transfection reagent volume
10 μL
Figure 25. siRNA transfection in HCT116 cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates that Booster achieves a stronger gene knockdown effect.
View official case PDF · page 17
HGC-27siRNA
Tumour cells
HGC-27 (Human Gastric Cancer Cells)
Open case link for HGC-27 (Human Gastric Cancer Cells), case 20 (opens in a new tab)
Case
20
Figure
Figure 26
Cell type
HGC-27
Nucleic acid type
siRNA
Culture plate size
T12
Nucleic acid amount
50 nM
Transfection reagent volume
10 μL
Figure 26. siRNA transfection in HGC-27 cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates that Booster achieves a stronger gene knockdown effect.
View official case PDF · page 17
RAW264.7Plasmid DNA(7000 bp)
Other common cell types
RAW264.7 (Mouse Monocyte/Macrophage Leukaemia Cells)
Open case link for RAW264.7 (Mouse Monocyte/Macrophage Leukaemia Cells), case 21 (opens in a new tab)
Case
21
Figure
Figure 27
Cell type
RAW264.7
Nucleic acid type
Plasmid DNA(7000 bp)
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
5 μL
Figure 27. Plasmid DNA transfection in RAW264.7 cells using Booster DNA&RNA Transfection Reagent. Western blot analysis shows significant overexpression of the target gene after transfection with Booster.
View official case PDF · page 18
RAW264.7siRNA
Other common cell types
RAW264.7 (Mouse Monocyte/Macrophage Leukaemia Cells)
Open case link for RAW264.7 (Mouse Monocyte/Macrophage Leukaemia Cells), case 22 (opens in a new tab)
Case
22
Figure
Figure 28
Cell type
RAW264.7
Nucleic acid type
siRNA
Culture plate size
6-well-plate
Nucleic acid amount
50 nM
Transfection reagent volume
5 μL
Figure 28. siRNA transfection in RAW264.7 cells using Booster DNA&RNA Transfection Reagent. The result demonstrates that Booster achieves a stronger gene knockdown effect.
View official case PDF · page 18
MC3T3-E1Plasmid DNA(5494bp)
Other common cell types
MC3T3-E1 (Mouse Embryonic Pre-Osteoblast Cells)
Open case link for MC3T3-E1 (Mouse Embryonic Pre-Osteoblast Cells), case 23 (opens in a new tab)
Case
23
Figure
Figure 29
Cell type
MC3T3-E1
Nucleic acid type
Plasmid DNA(5494bp)
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
3.75 μL
Figure 29. Plasmid DNA transfection in MC3T3-E1 cells using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 19
MC3T3-E1siRNA
Other common cell types
MC3T3-E1 (Mouse Embryonic Pre-Osteoblast Cells)
Open case link for MC3T3-E1 (Mouse Embryonic Pre-Osteoblast Cells), case 24 (opens in a new tab)
Case
24
Figure
Figure 30
Cell type
MC3T3-E1
Nucleic acid type
siRNA
Culture plate size
6-well-plate
Nucleic acid amount
50 nM
Transfection reagent volume
5 μL
Figure 30. siRNA transfection in MC3T3-E1 cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates that Booster achieves a stronger gene knockdown effect.
View official case PDF · page 19
AC16Plasmid DNA(1563bp)
Other common cell types
AC16 (Human Cardiomyocytes)
Open case link for AC16 (Human Cardiomyocytes), case 25 (opens in a new tab)
Case
25
Figure
Figure 31
Cell type
AC16
Nucleic acid type
Plasmid DNA(1563bp)
Culture plate size
12-well-plate
Nucleic acid amount
1 μg
Transfection reagent volume
2 μL
Figure 31. Plasmid DNA transfection in AC16 cells using Booster DNA&RNA Transfection Reagent.
View official case PDF · page 20
HaCaTPlasmid DNA(1500bp)
Other common cell types
HaCaT (Human Keratinocytes)
Open case link for HaCaT (Human Keratinocytes), case 26 (opens in a new tab)
Case
26
Figure
Figure 32
Cell type
HaCaT
Nucleic acid type
Plasmid DNA(1500bp)
Culture plate size
6-well-plate
Nucleic acid amount
2 μg
Transfection reagent volume
4 μL
Figure 32. Plasmid DNA transfection in HaCaT cells using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 20
DF-1Plasmid DNA(4700bp)
Other common cell types
DF-1 (Chicken Embryo Fibroblast Cells)
Open case link for DF-1 (Chicken Embryo Fibroblast Cells), case 27 (opens in a new tab)
Case
27
Figure
Figure 33
Cell type
DF-1
Nucleic acid type
Plasmid DNA(4700bp)
Culture plate size
24-well-plate
Nucleic acid amount
1 μg
Transfection reagent volume
1 μL
Figure 33. Plasmid DNA transfection in DF-1 cells using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 21
MFCsCo-transfection of plasmid DNA and mRNA
Other common cell types
MFCs (Mandarin Fish Cells)
Open case link for MFCs (Mandarin Fish Cells), case 28 (opens in a new tab)
Case
28
Figure
Figure 34
Cell type
MFCs
Nucleic acid type
Co-transfection of plasmid DNA and mRNA
Culture plate size
6-well-plate
Nucleic acid amount
Co-transfection of plasmid DNA and mRNA
Transfection reagent volume
3 μL transfection reagent + 2 μL transfection reagent
Figure 34. Co-transfection of plasmid DNA and mRNA in MFCs cells using Booster DNA&RNA Transfection Reagent versus L* 3000 transfection reagent.
View official case PDF · page 21
MACTPlasmid DNA(7500bp)
Other common cell types
MACT (Bovine Mammary Epithelial Cells)
Open case link for MACT (Bovine Mammary Epithelial Cells), case 29 (opens in a new tab)
Case
29
Figure
Figure 35
Cell type
MACT
Nucleic acid type
Plasmid DNA(7500bp)
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
7.5 μL
Figure 35. Plasmid DNA transfection in MACT cells using Booster DNA&RNA Transfection Reagent versus L*3000.
View official case PDF · page 22
HEK293Plasmid DNA(15000bp)
Other common cell types
HEK293 (Human Embryonic Kidney Cells)
Open case link for HEK293 (Human Embryonic Kidney Cells), case 30 (opens in a new tab)
Case
30
Figure
Figure 36
Cell type
HEK293
Nucleic acid type
Plasmid DNA(15000bp)
Culture plate size
12-well-plate
Nucleic acid amount
1 μg
Transfection reagent volume
2 μL
Figure 36. 15 Kb large fragment plasmid DNA transfection in HEK293 cells using Booster DNA&RNA Transfection Reagent. The results showed that Yeasen Booster transfection reagent had a significant overexpression effect.
View official case PDF · page 22
293TmRNA
Other common cell types
293T (Human Embryonic Kidney Cells)
Open case link for 293T (Human Embryonic Kidney Cells), case 31 (opens in a new tab)
Case
31
Figure
Figure 37
Cell type
293T
Nucleic acid type
mRNA
Culture plate size
6-well-plate
Nucleic acid amount
2.5 μg
Transfection reagent volume
2 μL
Figure 37. mRNA transfection in 293T cells using Booster DNA&RNA Transfection Reagent. The results showed that Yeasen Booster transfection reagent had a significant overexpression effect.
View official case PDF · page 23
293TPlasmid DNA(11000bp)
Other common cell types
293T (Human Embryonic Kidney Cells)
Open case link for 293T (Human Embryonic Kidney Cells), case 32 (opens in a new tab)
Case
32
Figure
Figure 38
Cell type
293T
Nucleic acid type
Plasmid DNA(11000bp)
Culture plate size
6-well-plate
Nucleic acid amount
5 μg
Transfection reagent volume
7.5 μL
Figure 38. 11 Kb large fragment plasmid DNA transfection in 293T cells using Booster DNA&RNA Transfection Reagent versus L*3000.
View official case PDF · page 23

Source: YEASEN official product page and linked case documents. These are manufacturer-published examples, not Direct Lab Supplies customer results.

Instructions, FAQs and references

Frequently asked questions

Is Booster suitable for both DNA and RNA?

YEASEN lists DNA, mRNA, siRNA, miRNA and ASO. This scope supports shortlisting the reagent, but does not establish identical performance for every payload or cell model.

Does every Booster application need the enhancer?

No. Manual EN20260916 includes it in the DNA workflow but states that it is not required for mRNA or siRNA. Follow that manual for the exact application.

Documents

Supporting documentMore Customer Cases -English Version Safety Data Sheet40801_MSDS_HB250715_EN.PDF ManualManufacturer manual · EN20260916 Certificate / COAInclude the batch number if known

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Product selection guides

Booster & RNAiBoost Transfection Guide
Explore Booster and RNAiBoost, their key features and manufacturer application data.
Buying YEASEN Reagents in the UK
Find product documents, request samples and arrange laboratory orders.
DNA vs siRNA Transfection: Reagent Guide
Compare DNA/RNA delivery and RNAi reagents by application and kit contents.
Reagent Pack Sizes: T, mL and Kit Components
Understand reaction counts, reagent volumes and supplied kit components.

Product comparisons

Lipofectamine 3000 Alternative UK
Looking for a Lipo 3000 alternative? Compare Yeasen Booster 40801 with Lipofectamine 3000: kit contents, pack sizes and GBP prices ex VAT.

Catalogue number

Pack size

Price ex VAT

Price incl. VAT

40801ES01

100 µL

£55.70

£66.84

40801ES03

0.75 mL

£264.67

£317.60

40801ES04

1.5 mL

£499.33

£599.20

Formulation

Polymer-based transfection reagent

Listed nucleic acids

DNA, mRNA, siRNA, miRNA and ASO

Supplied enhancer

Equal volume to Booster reagent

Enhancer use

DNA workflow; not required for mRNA or siRNA in manual EN20260916

Component code

Name

40801 ES 01

40801 ES 03

40801 ES 04

40801-A

Booster DNA/RNA Transfection Reagent

100 μL

0.75 mL

1.5 mL

40801-B

Transfection Enhancer

100 μL

0.75 mL

1.5 mL

Product selection guides

Booster & RNAiBoost Transfection Guide

Explore Booster and RNAiBoost, their key features and manufacturer application data.

Buying YEASEN Reagents in the UK

Find product documents, request samples and arrange laboratory orders.

DNA vs siRNA Transfection: Reagent Guide

Compare DNA/RNA delivery and RNAi reagents by application and kit contents.

Reagent Pack Sizes: T, mL and Kit Components

Understand reaction counts, reagent volumes and supplied kit components.