YeasenPCR & Genotyping

Hieff Canace™ Veritas High Fidelity Long PCR Master Mix (With Dye)

High-fidelity long PCR master mix with loading dye.

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Product information

Overview

Hieff Canace Veritas 10166 is a 2× high-fidelity long PCR master mix with dye. YEASEN reports 83× Taq and 9× standard Pfu fidelity in manual EN20260712, with blunt-ended products. See the manufacturer manual for supporting specifications.

Application: High-fidelity and long PCR
Format: 2× master mix with dye; blunt-ended products
Reported fidelity: 83× Taq; 9× standard Pfu — YEASEN manual EN20260712
Manufacturer-listed capability: 20–80% GC; 5–10 seconds/kb depending on fragment length

Features and manufacturer data

Hieff Canace Veritas 10166 is a 2× high-fidelity long PCR master mix with dye. YEASEN's current manual reports fidelity of 83× Taq and 9× standard Pfu, and blunt-ended amplification products. It describes a 20–80% GC range and extension of 5–10 seconds/kb depending on the fragment. These are manufacturer-reported specifications, not independent head-to-head results against other high-fidelity brands.

Manufacturer-reported fidelity: 83× Taq and 9× standard Pfu
5–10 seconds/kb extension, with length-dependent guidance
Manufacturer-listed GC range: 20–80%
Blunt-ended products and included electrophoresis loading dye

Manufacturer sources checked · 23 September 2026

Product page and linked manual checked on 23 September 2026. Manual revision: EN20260712. Manufacturer claims are not independent validation or a performance guarantee.

The download filename says EN20250822, but the actual PDF is revision EN20260712 and contains the 83× Taq / 9× Pfu statement.
The webpage's 20% crude-sample tolerance claim differs from the current manual's instruction to keep crude template at no more than 5% of the reaction. Follow the manual; a tolerance example is not a general operating instruction.
A supplier stability test at 37°C is not the recommended storage condition. Store this reagent frozen as specified.

Comparing another brand? Q5 PCR Alternatives UK: Compare Yeasen 10166.

Source: YEASEN product specifications. Product information recorded on 12 August 2026. Follow the current manufacturer instructions. Product documents and sources.

Applications, specifications and components

Applications: High-fidelity long PCR master mix with loading dye.
Sales pack: 250 µL · 1 mL · 5 × 1 mL · 25 mL
Catalogue options: 10166ES01, 10166ES03, 10166ES08, 10166ES30
Manufacturer: Yeasen
Manufacturer storage: Store at −25°C to −15°C for up to one year. Follow the expiry date on the supplied batch.

Catalogue numbers, pack sizes and prices

Catalogue number	Pack size	Price ex VAT	Price incl. VAT
10166ES01	250 µL	£14.86	£17.83
10166ES03	1 mL	£41.59	£49.91
10166ES08	5 × 1 mL	£166.35	£199.62
10166ES30	25 mL	£828.00	£993.60

Prices are in GBP. VAT-inclusive amounts use 20% VAT. Delivery is calculated at checkout. Select a pack size to check availability or request a quotation.

Specifications

Concentration	2×
Polymerase family	Canace high-fidelity DNA polymerase
Reported relative fidelity	83× Taq; 9× standard Pfu (YEASEN manual)
Product ends	Blunt
Manufacturer-listed GC range	20–80%
Extension guidance	5–10 seconds/kb, dependent on fragment length
cDNA amplification	Manufacturer webpage FAQ describes 1–14 kb; not a universal template limit

Components

Name	10166ES01	10166ES03	10166ES08	10166ES25	10166ES30
Hieff Canace™ Veritas Long PCR Master Mix (With Dye)	250 μL	1 mL	5×1 mL	25×1 mL	25 mL

Application data and case studies

Application figures supplied by YEASEN. These are manufacturer data, not Direct Lab Supplies customer results.

Figures

Manufacturer bar chart of mutation frequency for 80% GC gene sequencing, with bars labelled 10166, N*, K*, T* and Taq.

Figure1. High sensitivity of Hieff Canace™ High-Fidelity Long PCR Master Mix.

Figure 2. Long PCR from cDNA by Hieff Canace™ High-Fidelity Long PCR Master Mix.

Instructions, FAQs and references

Frequently asked questions

Is its fidelity really 83 times Taq?

YEASEN manual EN20260712 reports 83× Taq and 9× standard Pfu. This is a manufacturer-reported relative fidelity, not a claim of 83-fold higher yield, zero mutations or equivalence to Q5 or Phusion.

How long a fragment can it amplify?

YEASEN's product-page FAQ describes 1–14 kb cDNA amplification. The current manual includes guidance for fragments above 10 kb but does not set a universal maximum for every template type. Confirm your intended target if length is critical.

Is 5 seconds/kb the correct rate for every fragment?

No. The manual gives 5–10 seconds/kb and distinguishes fragments below 10 kb from those at or above 10 kb. Use its application-specific instructions rather than the fastest headline alone.

Can the website's 20% tolerance figure replace the manual?

No. The current manual limits crude-template addition to 5% of the total reaction. Contact YEASEN if you need to reconcile a different performance example with your intended use.

Notes

This product is for research use only.

Please operate with lab coats and disposable gloves，for your safety.

Citations and references

[1] Dong W, Zhu Y, Chang H, et al. An SHR-SCR module specifies legume cortical cell fate to enable nodulation. Nature. 2021;589(7843):586-590. doi:10.1038/s41586-020-3016-z (IF:42.779)
[2] Sun L, Yan Y, Lv H, et al. Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth. Cell Chem Biol. 2022;29(3):373-385.e6. doi:10.1016/j.chembiol.2021.10.006 (IF:8.116)
[3] Ye M, Xiong L, Dong Y, et al. The Potential Role of the Methionine Aminopeptidase Gene PxMetAP1 in a Cosmopolitan Pest for Bacillus thuringiensis Toxin Tolerance. Int J Mol Sci. 2022;23(21):13005. Published 2022 Oct 27. doi:10.3390/ijms232113005 (IF:6.208)
[4] He Y, Zhou J, Fu R, et al. The application of DNA-HRP functionalized AuNP probes in colorimetric detection of citrus-associated Alternaria genes. Talanta. 2022;237:122917. doi:10.1016/j.talanta.2021.122917 (IF:6.057)
[5] Guo L, Yang W, Huang Q, et al. Selenocysteine-Specific Mass Spectrometry Reveals Tissue-Distinct Selenoproteomes and Candidate Selenoproteins. Cell Chem Biol. 2018;25(11):1380-1388.e4. doi:10.1016/j.chembiol.2018.08.006 (IF:5.592)
[6] Dou W, Zhu Q, Zhang M, Jia Z, Guan W. Screening and evaluation of the strong endogenous promoters in Pichia pastoris. Microb Cell Fact. 2021;20(1):156. Published 2021 Aug 9. doi:10.1186/s12934-021-01648-6 (IF:5.328)
[7] Xiong Y, Yi Y, Wang Y, Yang N, Rudd CE, Liu H. Ubc9 Interacts with and SUMOylates the TCR Adaptor SLP-76 for NFAT Transcription in T Cells. J Immunol. 2019;203(11):3023-3036. doi:10.4049/jimmunol.1900556 (IF:4.718)
[8] Huang L, Xiang M, Ye P, Zhou W, Chen M. Beta-catenin promotes macrophage-mediated acute inflammatory response after myocardial infarction. Immunol Cell Biol. 2018;96(1):100-113. doi:10.1111/imcb.1019 (IF:4.557)
[9] Wang X, Du A, Yu G, Deng Z, He X. Guanidine N-methylation by BlsL Is Dependent on Acylation of Beta-amine Arginine in the Biosynthesis of Blasticidin S. Front Microbiol. 2017;8:1565. Published 2017 Aug 22. doi:10.3389/fmicb.2017.01565 (IF:4.076)
[10] Yu P, Zhou L, Yang WT, et al. Comparative mitogenome analyses uncover mitogenome features and phylogenetic implications of the subfamily Cobitinae. BMC Genomics. 2021;22(1):50. Published 2021 Jan 14. doi:10.1186/s12864-020-07360-w (IF:3.969)
[11] Liu G, Liu Y, Niu B, et al. Genetic mutation of TRPV2 induces anxiety by decreasing GABA-B R2 expression in hippocampus. Biochem Biophys Res Commun. 2022;620:135-142. doi:10.1016/j.bbrc.2022.06.079 (IF:3.575)
[12] Tan KS, Zhang Y, Liu L, et al. Molecular cloning and characterization of an atypical butyrylcholinesterase-like protein in zebrafish. Comp Biochem Physiol B Biochem Mol Biol. 2021;255:110590. doi:10.1016/j.cbpb.2021.110590 (IF:2.231)

Documents

Safety Data Sheet10166_MSDS_HB260630 ManualManufacturer manual · EN20260712 Certificate / COAInclude the batch number if known

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Product selection guides

PCR Mix Guide: Genotyping, Rapid & High Fidelity
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Understand YEASEN’s reported fidelity and the features of its long PCR mix.

Product comparisons

Q5 PCR Alternatives UK: Compare Yeasen 10166
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Catalogue number

Pack size

Price ex VAT

Price incl. VAT

10166ES01

250 µL

£14.86

£17.83

10166ES03

1 mL

£41.59

£49.91

10166ES08

5 × 1 mL

£166.35

£199.62

10166ES30

25 mL

£828.00

£993.60

Concentration

2×

Polymerase family

Canace high-fidelity DNA polymerase

Reported relative fidelity

83× Taq; 9× standard Pfu (YEASEN manual)

Product ends

Blunt

Manufacturer-listed GC range

20–80%

Extension guidance

5–10 seconds/kb, dependent on fragment length

cDNA amplification

Manufacturer webpage FAQ describes 1–14 kb; not a universal template limit

Name

10166ES01

10166ES03

10166ES08

10166ES25

10166ES30

Hieff Canace™ Veritas Long PCR Master Mix (With Dye)

250 μL

1 mL

5×1 mL

25×1 mL

25 mL

Hieff Canace™ Veritas High Fidelity Long PCR Master Mix (With Dye)