Which PCR master mix should you choose?

Genotyping, rapid or high-fidelity PCR: what differs?

Choose 10108 for genotyping, 10167 for rapid routine PCR, or 10166 for high-fidelity and long-fragment amplification. The mixes differ in polymerase, amplification conditions and the DNA ends they produce. Compare the details below with your downstream application.

Sources: YEASEN 10108 manual — EN20251208; YEASEN 10167 manual — body revision EN20260324; YEASEN 10166 manual — body revision EN20260712

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Is every PCR master mix a Taq mix?

10108 and 10167 contain Taq-based polymerases. 10166 contains Hieff Canace high-fidelity DNA polymerase.

For cloning or other downstream work, check whether you need 3′-A overhangs or blunt ends. Follow the protocol supplied for the selected mix.

Sources: Yeasen 10108 specifications; Yeasen 10167 specifications; Yeasen 10166 specifications

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Can you compare products by their fastest time or longest fragment?

Separate the fastest rate from the longest supported fragment. For 10167, the manual lists up to 15 kb but restricts the fastest 1 second/kb rate to specified short templates. The webpage gives different length figures in its features and table; the manual's 15 kb specification is not a guarantee for every template.

For 10166, the manual gives 5–10 seconds/kb with length-dependent guidance. Its webpage FAQ describes 1–14 kb cDNA amplification; that is not a universal maximum for all template types.

For 10108, manual EN20251208 confirms ligand-modified Taq with built-in Hot Start, resolving the contradictory ‘No’ entry in the website table. This guide uses the manual for the mechanism.

Sources: YEASEN 10108 manual — EN20251208; YEASEN 10167 manual — body revision EN20260324; YEASEN 10166 manual — body revision EN20260712; Yeasen 10166 specifications; Yeasen 10167 specifications

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Is a PCR mix with dye the same as a SYBR qPCR mix?

No. The dye in these endpoint PCR products supports the stated electrophoresis workflow. It does not turn them into a real-time fluorescence-detection formulation. If you need real-time PCR measurement, compare the separate qPCR range and the instrument requirements.

All three endpoint mixes listed here contain dye. Check compatibility before purchasing for an application that requires a colourless formulation.

Sources: Yeasen 10108 specifications; Yeasen 10167 specifications; Yeasen 10166 specifications

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Should you compare price per mL or price per reaction?

Our product pages show GBP pack prices excluding VAT and the price per mL or reaction where applicable. A 5 × 1 mL pack contains 5 mL; compare its price per mL with other packs of the same mix.

For prices per reaction, check the reaction volume stated by the manufacturer. Choose the formulation and pack size for your experiment, then compare the total cost including VAT and delivery.

• Intended endpoint application and required polymerase properties.
• Template type, expected fragment and downstream product-end requirement.
• Whether dye is acceptable in the supplied mix.
• Exact pack size, unit-price basis and required quantity.
• Current manual and any specification clarification needed before evaluation.

Sources: Direct Lab Supplies: current PCR packs and UK prices

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