UK laboratory buying guide
Q5 high-fidelity PCR alternatives: compare Yeasen 10166
YEASEN 10166 is a high-fidelity long PCR master mix with loading dye. Compare its format and manufacturer specifications with Q5 or Phusion, including the dye and DNA-end requirements of your application.
YEASEN products and GBP prices ex VAT
Compare pack sizes and current prices, or open a product for the full manufacturer details.
| Catalogue no. | Pack size | Price ex VAT | Select a pack |
|---|---|---|---|
| 10166ES01 | 250 µL | £14.86£59.44/mL£17.83 incl. VAT | View pack |
| 10166ES03 | 1 mL | £41.59£41.59/mL£49.91 incl. VAT | View pack |
| 10166ES08 | 5 × 1 mL | £166.35£33.27/mL£199.62 incl. VAT | View pack |
| 10166ES30 | 25 mL | £828.00£33.12/mL£993.60 incl. VAT | View pack |
Hieff Canace™ Veritas High Fidelity Long PCR Master Mix (With Dye)What matters when choosing
If you are comparing a Q5 Hot Start High-Fidelity 2× Master Mix with Yeasen 10166, the loading-dye format is an immediate difference. Q5 M0494 is colourless; Yeasen 10166 contains loading dye. Decide whether your priority is direct gel loading or a downstream application with its own dye requirements.
| Decision factor | Reference product | Yeasen option | Buying consideration |
|---|---|---|---|
| Separate enzyme or master mix | Q5 M0491S/M0491L supply a separate DNA polymerase with buffers. This is different from the Q5 M0494 master-mix family. | 10166 is a premixed PCR master mix with dye, not a standalone enzyme vial. | For M0491 searches, this is a different-format option for high-fidelity PCR, not an identical kit. Compare the complete contents before ordering.NEB Q5 High-Fidelity DNA Polymerase M0491NEB Q5 Hot Start High-Fidelity 2× Master Mix M0494Yeasen 10166: product specifications |
| Product format | Q5 M0494 is a 2× high-fidelity hot-start master mix. | Hieff Canace Veritas 10166 is a 2× high-fidelity long PCR master mix with dye. | A 2× format describes concentration, not identical enzymes, buffer chemistry or performance.NEB Q5 Hot Start High-Fidelity 2× Master Mix M0494Yeasen 10166: product specifications |
| Loading dye | M0494 is colourless. Q5 Quick-Load is a different product format. | 10166 includes electrophoresis indicators for direct gel loading. | For fluorescence, absorbance or other downstream work, check the chosen product's compatibility and purification requirements.NEB: colourless Q5 products and Quick-Load tracking dyesYeasen 10166: product specifications |
| Reference identity | M0494S, M0494L and M0494X are pack codes within the Q5 M0494 family. | Order the listed 10166ES retail pack that matches the volume you need. | Choose according to the required sequence accuracy, fragment length and downstream use. Similar pack volumes or product names do not demonstrate equal fidelity.NEB Q5 Hot Start High-Fidelity 2× Master Mix M0494Yeasen 10166: product specifications |
Reference products and catalogue numbers
Find your current product, then review the relevant YEASEN option. These products serve related applications but have different formulations and instructions.
- NEB Q5 Hot Start High-Fidelity 2X Master Mix
M0494S · M0494L · M0494X
- NEB Q5 High-Fidelity DNA Polymerase
M0491S · M0491L
- Thermo Scientific Phusion High-Fidelity PCR Master Mix with HF Buffer
F531S · F531L
- Invitrogen Platinum SuperFi II Green PCR Master Mix
12369250
Questions before switching products
What fidelity does YEASEN report for 10166?
Manual EN20260712 reports 83× Taq and 9× standard Pfu fidelity. These are YEASEN's relative-fidelity claims, not an independent comparison with Q5 or Phusion. Check the original manual and the separate fidelity explainer before interpreting the numbers.
Section linkIs Yeasen 10166 as accurate as Q5?
This guide does not establish that. Independent results under a shared measurement method would be needed for a comparative fidelity claim; separate manufacturer claims are not a like-for-like benchmark.
Section linkCan I carry over my existing Q5 method unchanged?
Do not assume that a different master mix has interchangeable instructions. Check the current Yeasen manual for the intended template and downstream application before evaluating it.
Section linkCan a pack price be converted into a universal cost per PCR?
No. Reaction volume and usable yield affect that calculation. Our product pages show the actual pack volume and GBP price ex VAT so your laboratory can budget against its own validated method.
Section linkManufacturer sources
We supply the Yeasen products listed above. This guide compares manufacturer specifications; it is not an independent performance study. Reference products are identified for comparison and are not offered for sale here.
Find product documents and sources, or read our UK buying guide for ordering and delivery information.
Consult the current product documents for your application.
- Yeasen 10166: product specifications
- YEASEN 10166 manual — body revision EN20260712
- NEB Q5 Hot Start High-Fidelity 2× Master Mix M0494
- NEB Q5 High-Fidelity DNA Polymerase M0491
- NEB: colourless Q5 products and Quick-Load tracking dyes
Products are for research use only. Third-party trade marks belong to their respective owners; no affiliation or endorsement is implied. Similar applications do not establish identical composition, performance or compatibility.
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