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SYBR Green qPCR master mix with blue tracking dye and a universal ROX reference formulation.
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Hieff UNICON Universal Blue is a 2× SYBR Green qPCR master mix for real-time PCR gene-expression analysis. The blue tracking dye and ROX passive reference dye have different functions; check the manufacturer guidance for your instrument.
Hieff UNICON Universal Blue is a 2× SYBR Green qPCR master mix containing antibody-mediated hot-start Taq, blue tracking dye and a universal ROX passive reference formulation. YEASEN describes balanced amplification across targets with 30–70% GC content. The fast programme uses a 20-second annealing/extension step; the standard programme uses 30 seconds. It is a dye-based qPCR mix, not a reverse-transcription kit or probe-specific mix.
Product page and linked manual checked on . Manual revision: EN20260609. Manufacturer claims are not independent validation or a performance guarantee.
Comparing another brand? PowerUp SYBR & Luna qPCR Alternatives UK.
Source: YEASEN product specifications. Product information recorded on . Follow the current manufacturer instructions. Product documents and sources.
| Catalogue number | Pack size | Price ex VAT | Price incl. VAT |
|---|---|---|---|
| 11184ES03 | 1 mL | £31.42 | £37.70 |
| 11184ES08 | 5 × 1 mL | £133.21 | £159.85 |
| 11184ES50 | 50 × 1 mL | £1,113.91 | £1,336.69 |
| 11184ES60 | 100 × 1 mL | £2,079.29 | £2,495.15 |
Prices are in GBP. VAT-inclusive amounts use 20% VAT. Delivery is calculated at checkout. Select a pack size to check availability or request a quotation.
| Concentration | 2× |
|---|---|
| Detection chemistry | SYBR Green I |
| Polymerase | Antibody-mediated hot-start Taq |
| Passive reference | Universal ROX formulation |
| Manufacturer-listed GC range | 30–70% |
| Annealing/extension step | 20 seconds (fast); 30 seconds (standard) |
| Component code | Name | 11184ES03 | 11184ES08 | 11184ES50 | 11184ES60 |
|---|---|---|---|---|---|
| 11184 | Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) | 1 mL | 5×1 mL | 50×1 mL | 100×1 mL |
Application figures supplied by YEASEN. These are manufacturer data, not Direct Lab Supplies customer results.
The current manual provides fast and standard programmes, with annealing/extension steps of 20 and 30 seconds respectively. Refer to the manual for instrument-specific guidance.
No. Blue dye is a visible loading aid; ROX is a passive fluorescence reference. Neither is the SYBR Green detection dye.
At the manual's 20 µL reaction format, 10 µL of 2× mix is used per reaction: 1 mL therefore provides 100 reactions before handling losses. A different reaction volume changes this count.
Compare specifications and instructions for your application before switching products.
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Manufacturer specificationsDye-based qPCR workflow reference with a universal passive reference dye.
Manufacturer specificationsFind product documents, request samples and arrange laboratory orders.
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Looking for a PowerUp SYBR Green or Luna qPCR alternative? Compare Yeasen 11184, reference dyes and kit features. GBP prices ex VAT.
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Unit prices exclude VAT.
Calculated using 10 µL of 2× mix per reaction, before handling losses. Other reaction volumes change this count.
YEASEN manual: 20 µL reaction format