Hifair™ AdvanceFast 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus)

Figures

1. Workflow: Fast & Simple

2. High-Efficiency Synthesis – Superior cDNA Yield Across Diverse Targets and Species

Using RNA from various sources, cDNA synthesis was carried out with different reverse transcription reagents following their respective protocols. Dye-based qPCR analysis demonstrated that 11155ES consistently produces higher cDNA yields and lower Ct values than other reagents, as reflected by the ∆Ct values.

3. High Sensitivity – Detects as Low as 10 pg of RNA

RNA from human 293T cells and Arabidopsis thaliana was serially diluted to different input amounts. cDNA synthesis was performed using various reverse transcription reagents according to protocols, followed by qPCR with Yeasen dye-based qPCR reagents. The ∆Ct [Ct (other reagents) – Ct (11155ES)] values indicate that, compared to other reverse transcription reagents, 11155ES consistently delivers better performance across a range of RNA inputs.

4. Enhanced gDNA Removal – More Effective Residual DNA Digestion

Genomic DNA (1000 ng) from human 293T cells was used as the template. Various reverse transcription reagents were tested following their respective protocols, with parallel groups set up without gDNA digestion. Reverse transcription was then performed, followed by qPCR using Yeasen 11185ES. The results indicate that the reagent effectively digests and removes residual gDNA.

5. Strong stability

Figure 5. Stability Test

(A) Performance after repeated freeze-thaw cycles. Reverse transcription was performed using reverse transcription reagents subjected to 0, 10, 20, or 30 freeze-thaw cycles, followed by dye-based qPCR. The resulting Cт values of samples subjected to 10–30 freeze-thaw cycles showed minimal variation compared to the control (0 cycles), with differences no greater than 0.5 Cт.

(B) Stability under different storage conditions. The stability of synthesized cDNA was evaluated after storage under various conditions. cDNA generated with product 11155ES was stored for 1 to 7 days at –80 °C, –20 °C, 4 °C, 25 °C, and 37 °C, respectively, prior to qPCR using Yeasen dye-based qPCR master mix.

The data indicate that Cт values obtained from samples stored under non–80 °C conditions were highly consistent with those stored at –80 °C, differing by no more than 0.5 Cт.

Frequently asked questions

Notes

1. This product is for research use only.

2. Please operate with lab coats and disposable gloves，for your safety.

3. Perform all pipetting and mixing steps on ice to maintain sample integrity.

4. Gently invert and centrifuge each component at low speed before use to ensure proper mixing.

Citations and references

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