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Three-colour prestained protein ladder spanning 10–245 kDa.
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GoldBand 20352 is a three-colour prestained protein ladder with 12 bands spanning 10–245 kDa, for SDS-PAGE and western blotting.
GoldBand 20352 is a ready-to-use three-colour prestained protein ladder with 12 bands from 10 to 245 kDa. It provides a 25 kDa green reference and a nominal 72 kDa orange reference, alongside blue bands. The manual supplies distinct Tris-glycine, Bis-Tris and HEPES-Tris calibrations for interpreting apparent molecular weight.
Product page and linked manual checked on . Manual revision: EN20250618. Manufacturer claims are not independent validation or a performance guarantee.
Comparing another brand? PageRuler Protein Ladder Alternatives UK.
Source: YEASEN product specifications. Product information recorded on . Follow the current manufacturer instructions. Product documents and sources.
| Catalogue number | Pack size | Price ex VAT | Price incl. VAT |
|---|---|---|---|
| 20352ES72 | 250μl | £51.99 | £62.39 |
| 20352ES76 | 2 × 250μl | £102.68 | £123.22 |
| 20352ES90 | 10 × 250μl | £390.29 | £468.35 |
Prices are in GBP. VAT-inclusive amounts use 20% VAT. Delivery is calculated at checkout. Select a pack size to check availability or request a quotation.
| Nominal range | 10–245 kDa |
|---|---|
| Band count | 12 |
| Nominal bands (kDa) | 10, 15, 20, 25, 35, 45, 60, 72, 100, 140, 180, 245 |
| Format | Three-colour prestained protein ladder |
| Component code | Name | 20352ES72 | 20352ES76 | 20352ES90 |
|---|---|---|---|---|
| 20352 | GoldBand™ 3-color High Range Protein Marker (10-245 kDa) | 250 μL | 2×250 μL | 10×250 μL |
Application figures supplied by YEASEN. These are manufacturer data, not Direct Lab Supplies customer results.
Attached table Under different electrophoresis buffer conditions, each band of this product indicates molecular weight
| Bands | colour | Tris-Glycine | Bis-Tris | Hepes-Tris |
|---|---|---|---|---|
| 1 | Blue | 245 | 240 | 235 |
| 2 | Blue | 180 | 173 | 175 |
| 3 | Blue | 140 | 135 | 136 |
| 4 | Blue | 100 | 94 | 98 |
| 5 | Red | 72 | 65 | 66 |
| 6 | Blue | 60 | 57 | 58 |
| 7 | Blue | 45 | 43 | 44 |
| 8 | Blue | 35 | 32 | 33 |
| 9 | Green | 25 | 23 | 24 |
| 10 | Blue | 20 | 19 | 20 |
| 11 | Blue | 15 | 14 | 15 |
| 12 | Blue | 10 | 10 | 10 |
62.5 mM Tris-H 3 PO 4 (pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300
1. After the product is thawed at room temperature,mix gently to fully dissolve the precipitate.
2. Then take an appropriate amount of this product into the gel hole. mini-gel: 3-5 μL; when the thickness of the gel is greater than 1.5 mm or the macromolecular bands need to be deepened, the loading volume can be appropriately increased: 5 - 10 μL.
0.1 - 0.4 μg/μL.
1. The product needs to be returned to room temperature before use to fully dissolve the precipitate. Incomplete protein denaturation at low temperature may lead to different degrees of dispersion of electrophoresis bands.
2. In western blot experiments, large proteins (>100 kDa) in the product may require longer transfer times or higher transfer voltages to complete transfer.
3. This product contains SDS, and the protein has been denatured, so it should not be used as a molecular weight reference standard for natural protein molecular electrophoresis.
4. The product will have deviations in protein size under different electrophoresis conditions, but after they are calibrated by non-prestained protein standards in the same buffer system, they can be used for protein determination of similar molecular weights.
5. At low concentration of gel, low molecular weight protein will swim on the dye front.
6. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents!
7. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety ！
8. For research use only!
Compare specifications and instructions for your application before switching products.
10–245 versus 10–250 kDa range; band set and migration differ.
Manufacturer specifications500 µL, 10–250 kDa reference; colour pattern and migration differ.
Manufacturer specificationsDiscontinued Imperial catalogue reference retained for search and cross-reference only; band pattern differs.
Manufacturer specificationsMulticolour broad-range protein standard; band set and apparent molecular weights differ.
Manufacturer specificationsChoose a prestained marker by molecular-weight range and gel system.
Compare Yeasen protein ladders with PageRuler, PageRuler Plus and SeeBlue Plus2 LC5925. Check band maps, pack sizes and GBP prices ex VAT.
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